Acidified QuEChERS method followed by GC-MS/MS on-cold injection for the determination of captan and folpet metabolites in strawberries and apple

Document Type : Original Article

Authors

1 Agricultural Research Center, Central Laboratory of Residue Analysis of Pesticides and Heavy Metals in Foods (QCAP), P.O. Box: 12311, Dokki, Giza, Egypt

2 Pesticide Research & Environmental Toxicology Section, Entomology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur-1701, Bangladesh

Abstract

In this work, the on-cold injection GC-MS/MS approach's development and evaluation for the simultaneous determination of both captan and folpet metabolites with endpoint backflush had been evaluated. An acidified QuEChERS approach for sample extraction, accompanied by the on-cold GC-MS/MS approach, provides an accurate quantification of captan and folpet metabolites in apple and strawberries at reporting limit (LOQ= 10µg/kg), which was lower than the EU-MRL and NAFSA-MRL. The method shows the analytical matrix effect, which was higher in apples (134%, 152%) than strawberries (83%, 115%) for captan and folpet metabolites tetrahydro phthalimide (THPI) and phthalimide (PI), consecutively. However, apple and strawberries correlation characteristics at three spiking levels of 10, 50, and 100µg/kg of both captan and folpet indicated a linear relationship (R2>0.99) which proves the reduction of the matrix effect. The recovery of captan metabolite ranged from 97% to122% and 80% to107%, while folpet metabolite ranged from 77% to104% and 78% to 111% for apple and strawberries, respectively, with RSD pooled <10%. The result confirms both method robustness and fits for their intended use that permits the practice in routine analysis.

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Egyptian Journal of Pure and Applied Science
Volume 61, Issue 3
October 2023
Pages 55-63

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